Fig. 6: RNF166 promotes CRC progression by regulating the Hippo pathway. | Cell Death & Disease

Fig. 6: RNF166 promotes CRC progression by regulating the Hippo pathway.

From: RNF166 promotes colorectal cancer progression by recognizing and destabilizing poly-ADP-ribosylated angiomotins

Fig. 6

A, B YAP target genes CTGF and CYR61 were detected by qPCR in stable RNF166-depleted and scramble CRC cells. C, D mRNA expression of CTGF and CYR61 was analyzed in FHC and HCT116 cells stably overexpressing RNF166. E, F AMOTL2 increasement and YAP inactivation were observed in CRC cells with stable knockdown of RNF166 by immunoblotting. G Immunofluorescence analysis and quantitative analysis of the YAP distribution in HCT116 cells with stable knockdown of RNF166. H Luciferase activity was detected in the 293 T cells by transfecting RNF166 or empty vector plasmids and luciferase reporter. I IHC of serial sections showed consistent expression of RNF166 and YAP in human CRC tissues and tumors. Scale bar, 50 μm. J H&E and IHC analysis of RNF166, YAP and Ki-67 levels in scramble or RNF166-depleted xenograft tumors. Scale bar, 50 μm. For (A–D) and (H), **p < 0.01, ***p < 0.001, and ****p < 0.0001.

Back to article page