Fig. 1: POLQ positively regulates stem cell-like characteristics of gastric cancer cells. | Cell Death & Disease

Fig. 1: POLQ positively regulates stem cell-like characteristics of gastric cancer cells.

From: POLQ inhibition attenuates the stemness and ferroptosis resistance in gastric cancer cells via downregulation of dihydroorotate dehydrogenase

Fig. 1

A The levels of POLQ were detected in adherent cells and spheres derived from AGS and MGC-803 cells using qRT-PCR. B POLQ protein levels in AGS and MGC-803 cells expressing shRNA or shPOLQ. C Cell proliferation of control and POLQ- knockdown cell lines were determined using CCK-8 assay. D Public database [https://depmap.org/portal/] correlation analysis in cell lines (n = 84) between POLQ and MKI67. Significance was evaluated by Pearson correlation. E The mRNA levels of MKI67 were detected in control and POLQ- knockdown cell lines. The mRNA levels of gastric cancer stem cell markers (F) and differentiated cell markers (G) in control and POLQ- knockdown cell lines. H A series of limiting diluted POLQ-knockdown cells and their corresponding control cells were subcutaneously inoculated into nude mice. Bioluminescent imaging (BLI) was performed. I The CSC frequency was calculated using ELDA software. Results are shown as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.

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