Fig. 2: POLQ regulates the cancer stem cell-like characteristics of GC cells through DHODH. | Cell Death & Disease

Fig. 2: POLQ regulates the cancer stem cell-like characteristics of GC cells through DHODH.

From: POLQ inhibition attenuates the stemness and ferroptosis resistance in gastric cancer cells via downregulation of dihydroorotate dehydrogenase

Fig. 2

A Heatmap showing differently expressed genes in control and POLQ-knockdown GC cells. Values are scaled as indicated (2 to −2; n = 3). B Heatmap showing differently expressed genes in POLQlow versus POLQhigh stomach adenocarcinoma. C Public database [https://depmap.org/portal/] correlation analysis in cell lines (n = 84) between POLQ and DHODH. Significance was evaluated by Pearson correlation. D, E The mRNA and protein levels of DHODH were detected in control and POLQ- knockdown cell lines. F, G The mRNA and protein levels of DHODH were detected in GC cells treated with vehicle or NVB (100 µM) for 24 h. H Protein expression levels in POLQ- knockdown cells overexpressing DHODH. I Sphere formation in control (ShNC), POLQ knockdown (ShPOLQ), and ShPOLQ + DHODH cells was analyzed using oncosphere-initiating medium. J The serial sphere-forming capacity. K The mRNA levels of gastric cancer stem cell markers in control (ShNC), POLQ knockdown (ShPOLQ), and ShPOLQ + DHODH cells. Results are shown as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.

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