Fig. 6: Pharmacological inhibition of POLQ attenuates the stemness and ferroptosis resistance in gastric cancer cells.

A The mRNA levels of gastric cancer stem cell markers in GC cells treated with vehicle or NVB (100 µM) for 24 h. B Cell viability in GC cells treated with vehicle, RSL3, erastin or combined NVB for 24 h. C Analysis of combination of RSL3 and NVB in GC cells using combenefit software. D The mRNA levels of PTGS2 were detected in GC cells treated with RSL3, erastin or combined NVB for 24 h. E, F Respective lipid peroxidation levels were assessed and calculated by flow cytometry using BODIPY C11. G The protein levels of 4HNE were detected in GC cells treated with RSL3, erastin or combined NVB for 24 h. H MGC-803 cells were incubated with vehicle, 1-stearoyl-2-15(S)-HpETE-sn-glycero-3-PE or combined NVB for 24 h and stained with Calcein AM reagent and the representative images were acquired by fluorescence microscope (scale bar = 500 μm). I, J Respective lipid peroxidation levels were assessed and calculated by flow cytometry using BODIPY C11. K Left: BLI of the metastatic burden of mice intravenously injected with MGC-803-luc cells. The mice were treated with vehicle, NVB or combined Liproxstatin-1. Right: The proportion of pulmonary metastases. Results are shown as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.