Fig. 1: FOXP3 linked to ferroptosis regulation and elevated in GBM tissues. | Cell Death & Disease

Fig. 1: FOXP3 linked to ferroptosis regulation and elevated in GBM tissues.

From: FOXP3 promote the progression of glioblastoma via inhibiting ferroptosis mediated by linc00857/miR-1290/GPX4 axis

Fig. 1

A An RNAi screen was performed using an shRNA library targeting 45 members of the FOX family to comprehensively identify specific FOXs that have the potential to regulate ferroptosis in GBM cells. B Volcano indicated that the depletion of FOXP3, FOXO1, FOXA2, and FOXM1 resulted in an increased sensitivity of U87 cells to erastin, whereas inhibition of FOXQ1 and FOXO3 led to a reduction in sensitivity. C High levels of FOXP3 was observed in GBM tissues from TCGA database. D High levels of FOXP3 predicted lower survival days in patients with GBM from TCGA database. E qRT-PCR was used to detect the expression of FOXP3 in GBM tissues and non-tumor brain tissues from research cohort. F IHC was used to detect the expression of FOXP3 in GBM tissues and non-tumor brain tissues from research cohort. G, H qRT-PCR and western blotting was used to detect the expression of FOXP3 in NHA, T98, U87, A172 and LN229 cell. I immunofluorescence analysis demonstrated that FOXP3 predominantly localized within the cell nucleus. **P < 0.01.

Back to article page