Fig. 2: FOXP3 increased the proliferation of GBM cells via inhibiting ferroptosis.

The GSH (A), MDA (B), Iron (C), GPX (D), and ROS level (E) was detected in U87 and LN229 cells with FOXP3-knockdown or FOXP3-overexpression. F Western blotting was used to detect the expression of FOXP3, GPX4, SLC40A1, SLC7A11, and FTH1 in U87 and LN229 cells with FOXP3-knockdown or FOXP3-overexpression. G CCK-8 indicated the effects of FOXP3-overexpression and FOXP3-knockdown on U87 and LN229 proliferation. H Colony formation indicated the effects of FOXP3-overexpression and FOXP3-knockdown on U87 and LN229 colony formation. I EDU assay indicated the change of EDU positive rate after FOXP3-overexpression and FOXP3-knockdown in U87 and LN229. *P < 0.05; **P < 0.01.