Fig. 5: Linc00857 increased the expression of GPX4 via sponging miR-1290. | Cell Death & Disease

Fig. 5: Linc00857 increased the expression of GPX4 via sponging miR-1290.

From: FOXP3 promote the progression of glioblastoma via inhibiting ferroptosis mediated by linc00857/miR-1290/GPX4 axis

Fig. 5

A qRT-PCR was used to detect the mRNA levels of GPX4 after linc00857 knockdown. B, C Subcellular localization of linc00857 was measured using FISH or nucleocytoplasmic fractionation RT-qPCR. D Potential miRNAs regulated by linc00857 which can bind with GPX4 were predicted by indicated websites. E Expression of potential miRNAs regulated by linc00857 were detected by RT-qPCR in cells with linc00857 knockdown. F miR-1290 and GPX4 were detected with RT-qPCR in cells with miR-1290-overexpression. G Predicted binding sites between linc00857, GPX4, and miR-1290. H, I Luciferase activation was detected in cells co-transfected with miR-1290 and wildtype/mutation 3′UTR of linc00857/GPX4. J RIP assay was conducted to confirm the interaction between AGO2, linc00857, miR-1290 and GPX4. K The co-expression relationship between FOXP3, linc00857, miR-1290 and GPX4 using ISH and IHC method. **P < 0.01.

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