Fig. 2: TGM3 inhibits cSCC cell proliferation, migration and invasion in vitro, and suppresses tumor growth in vivo. | Cell Death & Disease

Fig. 2: TGM3 inhibits cSCC cell proliferation, migration and invasion in vitro, and suppresses tumor growth in vivo.

From: Transglutaminase 3 regulates cutaneous squamous carcinoma differentiation and inhibits progression via PI3K-AKT signaling pathway-mediated Keratin 14 degradation

Fig. 2

A Effects of TGM3 knockdown and overexpression on cSCC cell proliferation. CCK-8 assays were performed with the stable cells above. n = 5. B Effects of TGM3 overexpression on cSCC cell colony formation. Relative colony numbers were counted and shown in the right panel. n = 5. C Effects of TGM3 knockdown on cSCC cell colony formation. Relative colony numbers were counted and shown in the right panel. n = 5. D Effects of TGM3 overexpression on cSCC cell migration and invasion. E Effects of TGM3 knockdown on cSCC cell migration and invasion. F Relative cell numbers in SCL-1 (left panel) and A431 cells (right panel). n = 5; Scale bar, 100 μm. G Quantification of tumor weight (left panel) and tumor volume (right panel) of subcutaneous A431 xenograft tumor for each group. n = 5. All experiments were repeated three or five times. Results were expressed as mean ± SD; n.s. not significant; *P < 0.05; **P < 0.01; ***P < 0.001; Two way ANOVA Sidak test (A left panel); Two way ANOVA Dunnett test (A right panel; G right panel); Unpaired two-tailed t test (B; C; F, left panel; 2G, left panel); Welch’s test (F, left panel; G, left panel); Brown-Forsythe and Welch ANOVA tests (C; F, right panel); Mann-Whitney (2 F, right panel).

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