Fig. 6: Loss of TGM3 associates with increased AKT activity and accelerated KRT14 in human cSCC tissues. | Cell Death & Disease

Fig. 6: Loss of TGM3 associates with increased AKT activity and accelerated KRT14 in human cSCC tissues.

From: Transglutaminase 3 regulates cutaneous squamous carcinoma differentiation and inhibits progression via PI3K-AKT signaling pathway-mediated Keratin 14 degradation

Fig. 6

A Representative IHC staining intensity of AKT phosphorylation (Ser473) in cSCC and adjacent non-tumor tissue. Scale bar, 200 μm. B Representative IHC staining intensity of KRT14 in cSCC and adjacent non-tumor tissue. Scale bar, 200 μm. C Scores of AKT phosphorylation (Ser473) IHC staining in cSCC and adjacent non-tumor tissue. n = 5. D Scores of KRT IHC staining in cSCC and adjacent non-tumor tissue. n = 5. E Representative images of H&E staining and IHC staining. The IHC staining of cSCC and adjacent non-tumor tissue demonstrated that TGM3 displayed an opposing pattern to AKT phosphorylation (Ser473) and KRT14.Scale bar, 200 μm. F The negative correlations between TGM3 protein level and AKT phosphorylation (Ser473) and KRT14 protein level in human cSCC and adjacent non-tumor tissue. n = 48. All experiments were repeated at least three times. Results were expressed as mean ± SD; n.s. not significant; **P < 0.01; ***P < 0.001; Welch’s test (C; D); Pearson correlation analysis (F).

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