Fig. 6: MSF-2 subgroup consists of activated fibroblasts.
From: Sprouty genes regulate activated fibroblasts in mammary epithelial development and breast cancer
A–C mRNA expression as detected by qPCR of several genes in the marker panel of the MSF-1 (A), MSF-2 (B), and MSF-4 (C) to validate whether subgroup cells have been specifically enriched by FACS. Expr, expression. D–F mRNA expression as detected by qPCR of Fgf2 (D) and Fgf10 (E) or several RTK signaling target genes (F) in the MSF-1, MSF-2, and MSF-4 subgroups to validate whether subgroup cells have been specifically enriched by FACS. G, H Wildtype organoids co-cultured with fibroblasts from the MSF-1 to MSF-4 subgroups (H) with their branching ratios quantified (I). N = 3. Scale bars: 50 μm. Data were from three independent experiments. Plots show mean ± SD (n \(\ge\)3); ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Scale bars: 50 μm. I–L Confocal images of immunofluorescence of PDGFR (I, J), K14 (I’, K’, J’, L’), CD36 (K, L), and overlay, marking pan-fibroblasts and MSF-2 fibroblasts, respectively, around mammary ducts (I, K) or TEBs (J, L) using frozen sections of mammary glands at the 7-week stage. Note blue nuclei in the overlayed images. Scale bars: 20 μm.
