Fig. 5: FBXL8 targets Snail1 for ubiquitin-proteasome degradation. | Cell Death & Disease

Fig. 5: FBXL8 targets Snail1 for ubiquitin-proteasome degradation.

From: FBXL8 inhibits post-myocardial infarction cardiac fibrosis by targeting Snail1 for ubiquitin-proteasome degradation

Fig. 5

A, B The mRNA, protein level (A), and quantification (B) of Snail1 mediated by FBXL8 overexpression (n = 3). C–F CFs transfected with Flag-vector, Flag-FBXL8 plasmids (C, D), control shRNA, or FBXL8 shRNA (E, F) were treated with 40 μg/mL CHX for the indicated periods and then analyzed by Western blotting (n = 3). G, H Western blotting (G) and quantification (H) of Snail1 in CFs following HA-FBXL8 overexpression for 24 h and stimulated with TGFβ for another 36 h, MG-132 (50 μM) or Bafilomyocin A1 (50 μM) for 6 h (n = 3). I Western blot of ubiquitination assays assessing the ubiquitin level of Snail1 after FBXL8 overexpression or knockdown for 24 h and treated with MG-132 (50 μM) for 6 h in HEK293T cells. RCF rat cardiac fibroblast, HDF human dermal fibroblast, HLF human lung fibroblast. Data are presented as mean ± SD. *P < 0.05.

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