Fig. 6: Snail1 supplement abolishes FBXL8-regulated myofibroblast differentiation. | Cell Death & Disease

Fig. 6: Snail1 supplement abolishes FBXL8-regulated myofibroblast differentiation.

From: FBXL8 inhibits post-myocardial infarction cardiac fibrosis by targeting Snail1 for ubiquitin-proteasome degradation

Fig. 6

A–C Representative western blots (A) and quantification (B, C) of α-SMA, Col1a1, active RhoA, total RhoA, FBXL8, and Snail1 protein levels in cardiac fibroblasts transfected with FBXL8 or FBXL8 + Snail1 for 24 h and then stimulated by TGFβ (10 ng/ml) for another 36 h (n = 3). D, E Immunofluorescence (D) and quantitation (E) of α-SMA in CFs (n = 5). F, G Representative image (F) and quantification (G) of migrated cells in the indicated groups (n = 5). H, I Representative image (H) and quantification (I) of gel contraction in the indicated groups (n = 5). Data are presented as mean ± SD. *P < 0.05.

Back to article page