Fig. 6: ASS1 inhibits the proliferation of TNBC cells mainly by inhibiting serine synthesis. | Cell Death & Disease

Fig. 6: ASS1 inhibits the proliferation of TNBC cells mainly by inhibiting serine synthesis.

From: ASS1 inhibits triple-negative breast cancer by regulating PHGDH stability and de novo serine synthesis

Fig. 6

A Hs578T cells stably expressing the control empty plasmid or Flag-ASS1, were deprived or not of serine and glycine and subjected to MTT assays. Four independent experiments were performed and data are shown as the means ± SD with p-value based on two-way ANOVA (n = 4, **p < 0.01, ***p < 0.001). B MDA-MB-468 cells stably expressing the control empty plasmid or Flag-ASS1, were deprived or not of serine and glycine and subjected to MTT assays. Four independent experiments were performed and data are shown as the means ± SD with p-value based on two-way ANOVA (n = 4, **p < 0.01, ***p < 0.001). C, D MDA-MB-468 cells stably expressing the control empty plasmid or Flag-ASS1, were deprived or not of serine and glycine and subjected to colony formation assays. Representative images and quantitative results of colonies are shown. Three independent experiments were performed and data are shown as the means ± SD with p-value based on a two-way ANOVA (n = 3, **p < 0.01, n.s., not significant). E Hs578T cells with or without depletion of serine and glycine and ASS1 knockdown cells (KD#1 and KD#2) depleted of serine and glycine were subjected to MTT assays. Four independent experiments were performed and data are shown as the means ± SD with p-value based on two-way ANOVA (n = 4, *p < 0.05, **p < 0.01). F MDA-MB-468 cells with or without depletion of serine and glycine and ASS1 knockout cells (KO#1 and KO#2) depleted of serine and glycine were subjected to MTT assays. Four independent experiments were performed and data are shown as the means ± SD with p-value based on two-way ANOVA (n = 4, **p < 0.01, ***p < 0.001). G, H MDA-MB-468 cells with or without depletion of serine and glycine and ASS1 knockout cells (KO#1 and KO#2) depleted of serine and glycine were subjected to colony formation assays. Representative images and quantified results of colonies are shown. Three independent experiments were performed and data are shown as the means ± SD with p-value based on two-way ANOVA (n = 3, *p < 0.05, **p < 0.01, n.s., not significant). I Hs578T cells stably expressing the control empty plasmid or Flag-ASS1 supplemented with or without 10 mM serine and glycine in normal media were subjected to MTT assays. Four independent experiments were performed and data are shown as the means ± SD with p-value based on a two-way ANOVA (n = 4, **p < 0.01). J MDA-MB-468 cells stably expressing the control empty plasmid or Flag-ASS1 supplemented with or without 10 mM serine and glycine in normal media were subjected to MTT assays. Four independent experiments were performed and data are shown as the means ± SD with p-value based on two-way ANOVA-test (n = 4, **p < 0.01). K, L MDA-MB-468 cells stably expressing the control empty plasmid or Flag-ASS1 supplemented with or without 10 mM serine and glycine in normal media were subjected to colony formation assays. Representative images and quantified results are shown. Three independent experiments were performed and data are shown as the means ± SD with p-value based on a two-way ANOVA (n = 3, **p < 0.01).

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