Fig. 2: Overexpression of HNF1A-AS1 promotes self-renewal of GCSCs in vitro.
From: CMYC-initiated HNF1A-AS1 overexpression maintains the stemness of gastric cancer cells
A Colony formation assays were used to explore the cell colony formation ability of LV-HNF1A-AS1 and LV-NC-transfected cells. B The bar graph showed the number of gastric cancer cell clones formed (n = 3). C Representative images of GCSC spheres overexpressing LV-HNF1A-AS1. Original magnification, ×40. D, E The bar graph showed the number of spheres formed (n = 3). F, G CCK8 assay to investigate the effect of overexpression of HNF1A-AS1 on the proliferative capacity of GC cells (n = 3). H, I qRT-PCR analysis of the expression of HNF1A-AS1 in MKN-45 and BGC-823 cells after 12 h, 2, 4, 6 and 10 d of spheroid formation (n = 3). Data are representative as the mean ± SD. Two-tailed unpaired Student’s t-test (B, D, E), two-way ANOVA test (F, G), one-way ANOVA with Tukey’s multiple-comparison test (H, I). *P < 0.05; **P < 0.01; ***P < 0.001.
