Fig. 2: Western blot on SEE-stimulated T blasts. | Cell Death & Disease

Fig. 2: Western blot on SEE-stimulated T blasts.

From: Study of the potential role of CASPASE-10 mutations in the development of autoimmune lymphoproliferative syndrome

Fig. 2

A Western blot on SEE-stimulated T blasts generated from healthy controls, included subjects and ALPS-FAS patients with mutations in FAS extracellular (ECD) or intracellular (ICD) domain. Raji cell lines were used as negative controls due to their lack of Caspase-10 expression, while Ku70 (housekeeping protein) was used as loading control. The most expressed Caspase-10 isoforms (uncleaved Caspase-10A and D) are shown. B Quantitative determination of Caspase-10 protein levels normalized with respect to Ku70 levels. Western blot is representative of one experiment from n = 1 sample of SEE T blasts for each individual. HMZ homozygous, HTZ heterozygous, Ctrl control, kDa kiloDalton.

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