Fig. 2: The combination of BMP4 and DBZ strongly induces the expression of enterocytic and mucosecretory markers. | Cell Death & Disease

Fig. 2: The combination of BMP4 and DBZ strongly induces the expression of enterocytic and mucosecretory markers.

From: Vitamin D opposes multilineage cell differentiation induced by Notch inhibition and BMP4 pathway activation in human colon organoids

Fig. 2

A Light microscopy images of colon normal organoids cultured in PROL, DIFF, or B + D media for 72 h (patient #110). Scale bar, 500 μm. B RT-qPCR analysis of the RNA levels of stemness, enterocytic and mucosecretory genes in organoids from patients #47, #86, #110, #130 for 48 h and #47, #110, #130, #159 for 72 h cultured in PROL (black), DIFF (red) or B + D (green) media. *P < 0.05, **P < 0.01, ***P < 0.001. C Immunofluorescence analysis of the expression of enterocytic and mucosecretory protein markers (green) in organoids cultured for 48 h in PROL or B + D media (patient #158). Scale bars, 30 µm. Box-plots show the quantification of the fluorescence intensity (Log2 MGV) (patients #92, #158 and #166; the number of organoids analyzed was 55 for KLF4, 80 for KRT19, 83 for AGR2 and 79 for TFF2). **P < 0.01, ***P < 0.001. MGV: mean gray value.

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