Fig. 2: Lipidomics analysis identifies changes in free cholesterol and cholesterol esters upon C10 treatment.
From: Cytotoxic sigma-2 ligands trigger cancer cell death via cholesterol-induced-ER-stress
Bar graphs showing levels of free cholesterol (a) and cholesterol esters (b) in cells treated with the indicated dose of C10 for 24 h (n = 3), or KP2 tumor samples derived from C57BL6 mice after treatment with C10 [60 mg/kg] for 3 days via intraperitoneal injection (n = 4). Data are presented as means ± SD. Heatmap summarizing the statistical analysis of (a, b). The white boxes marked with X indicate that the metabolite was not analyzed (i.e., CE(22:6) in the in vivo model). c Representative confocal microscopy images from MiaPaCa-2 treated with DMSO, U18666a, or C10 for 24 h, showing the merged image of blue and [yellow] channels from filipin and [Nile Red], measuring free cholesterol and [cholesterol esters] respectively. Scale bar equals 20 µm. d Cell death over time of AsPC-1 cells treated with a serial dilution of mevalonate (DF = 1.5) alone [left] or in combination with a sublethal dose of C10 [right]. Data represent mean ± SD; n = 3. ref. reference, ns not significant; *p < 0.05; **p < 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.
