Fig. 6: FOXP4’s oncogenic potential in OV cells requires PTK7. | Cell Death & Disease

Fig. 6: FOXP4’s oncogenic potential in OV cells requires PTK7.

From: FOXP4-mediated induction of PTK7 activates the Wnt/β-catenin pathway and promotes ovarian cancer development

Fig. 6

A PTK7 was retrovirally infected into A2780 cells stably expressing FOXP4-shRNA. Lysates of A2780 cells with pLKO, sh-FOXP4, and sh-FOXP4 + PTK7 were immunoblotted with the indicated antibodies. B The cell growth curve of cells from (A), n = 3. **p < 0.01. C Incorporation of BrdU in cells from (A) was measured by ELISA, n = 3. **p < 0.01, ***p < 0.001. D Clonogenic assay of cells in (A), n = 3. *p < 0.05, **p < 0.01, ***p < 0.001. E Relative migration rates of cells in (A) in a transwell assay, n = 3. **p < 0.01, ***p < 0.001. F A2780 cells stably expressing pLKO or sh-FOXP4 and stably expressing sh-FOXP4 + PTK7 were inoculated subcutaneously in nude mice, respectively, and the folded line graphs indicate the average volume of tumors over time, n = 6. ***p < 0.001. G Image representing the tumor on day 28, n = 6. H Bar graphs represent the weight of the tumor on day 28, n = 6. ***p < 0.001.

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