Fig. 3: LMW HA activates non-canonical IRAK1 signaling and stemness. | Cell Death & Disease

Fig. 3: LMW HA activates non-canonical IRAK1 signaling and stemness.

From: Selective targeting of IRAK1 attenuates low molecular weight hyaluronic acid-induced stemness and non-canonical STAT3 activation in epithelial ovarian cancer

Fig. 3

A Western blot for IRAK1 across EOC cell lines. B Western blot time course for pIRAK1 (T209) and total IRAK1 in A1847 and OVCAR8 cells following stimulation with LMW HA (200 ng/mL). GAPDH served as an internal control. C Representative images of spheroid formation assay in A1847 and OVCAR8 cells either unstimulated or stimulated with LMW HA for 14 days. D Quantification of (C). Data is represented as mean from three independent experiments ± SEM. E (Top) Representative image of low and high exposures of immunoassay-based kinase array following stimulation with or without LMW HA for 15 min. 1. pSTAT5; 2. pp38; 3. β-Catenin; and 4. pERK. (Bottom) Quantification of pixel density of relative to untreated control. F Western blot of A1847 and OVCAR8 time course assessing phosphorylated and total expression of p38 and STAT3 following stimulation with or without LMW HA. The STAT3 target gene, MYC, was also assessed. *p < 0.05, ****p < 0.0001.

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