Fig. 4: IRAK1 is critical for HGSOC growth and LMW HA-induced stemness.

A Western blot of IRAK1 in IRAK1 KD and IRAK1 Scr cells and phosphorylated and total p65 and p38. B Heatmap of RNA-sequencing comparing IRAK1 KD and Scr gene expression. C Volcano plot of RNA-seq data from (B). Genes associated with stemness are labeled. D Western blot KD and Scr for pSTAT3, total STAT3, and cleaved Notch1 and Notch3. E Representative image of CF comparing KD and Scr. F Quantification of (E) for (i) colony number and (ii) colony size. Data are represented as mean from three independent experiments ± SEM. G Representative images of Scr and KD spheroid formation. H Quantification of spheroid formation from (G). Data are represented as box plots, min to max, all data points from three independent experiments. I Representative image of NSG mice injected IP with either IRAK1 Scr or KD cells. J Quantification of malignant ascites volume from IRAK1 Scr and KD groups (n = 5 mice per group). Data represented as mean ± SEM showing all data points. K Quantification of tumor weight following microdissection from IRAK1 Scr and KD groups (n = 5 mice per group). Data represented as mean ± SEM showing all data points. L Western blot time course of IRAK1 Scr and IRAK1 KD cells stimulated with LMW HA for phosphorylation and total STAT3. GAPDH served as internal control. M representative images of spheroid formation for IRAK1 Scr and KD cells stimulated with or without LMW HA for 14 days. N Quantification of spheroid number from (M). Data represented as mean ± SEM from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant.