Fig. 5: TCS2210 is a selective inhibitor of IRAK1. | Cell Death & Disease

Fig. 5: TCS2210 is a selective inhibitor of IRAK1.

From: Selective targeting of IRAK1 attenuates low molecular weight hyaluronic acid-induced stemness and non-canonical STAT3 activation in epithelial ovarian cancer

Fig. 5

A Chemical structure of TCS2210. B In silico docking of TCS2210 with IRAK1: (i) space-filled model (ii) zoomed-in space-filled model with predicted hydrogen bonding interactions. C Kinome tree interaction map of Eurofins ScanMAX analysis with TCS2210. D Western blot of CETSA assay for IRAK1 following incubation with TCS2210 or DMSO vehicle control. E SPR analysis of TCS2210 with active recombinant IRAK1 enzyme. Data are shown for 1:1 kinetic model and 1:1 affinity model. F Western blot of A2780 cells for pIRAK1 (T209) and total IRAK1 following preincubation with or without TCS2210, and stimulation with or without LMW HA.

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