Fig. 1: Interference with TRIM65 inhibited the proliferation of renal cell carcinoma cells.

A The expression of TRIM65 protein in four renal cell carcinoma cell lines and control HK-2 cells. B CCK8 assay. Cells were infected with lentivirus TRIM65 shRNA-1/shRNA-2 or control to generate stable cell lines. Then CCK8 cell proliferation assay was performed using those stable cells. C Colony formation assay. 10–14 days later, the indicated cells grown in 6-well plates were fixed and stained with 2% crystal violet. D The number of colonies was counted and data are presented as mean ± SEM (n = 3). E Soft agar colony formation assay. The above stable RCC cells were seeded in 6-well plates. 15–20 days later, cells were imaged, colonies larger than 50 μm were counted, and calculated the area. F The number of cell colonies was calculated in eight randomly selected views. Data are presented as mean ± SEM (n = 8). G The average area of colonies was calculated in eight randomly selected views. Data are presented as mean ± SEM (n = 8). *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t-test.