Fig. 3: TRIM65 interacted with BTG3.
From: TRIM65 promotes renal cell carcinoma through ubiquitination and degradation of BTG3
A BTG3 was identified as a new binding protein of TRIM65 via the yeast-two-hybrid system. B Co-immunoprecipitation analysis of the interaction between FLAG-TRIM65 and HA-BTG3. C Endogenous interaction of TRIM65 and BTG3. D The purified GST or GST-TRIM65 protein was incubated with the lysates of BTG3-transfected HEK293T cells. E Co-localization of TRIM65 and BTG3 in RCC cells. ACHN cells were co-stained with antibodies against BTG3 and TRIM65 with or without MG132. F Interaction of BTG3 with GFP-TRIM65 and its deletion mutants was determined. G Schematic diagram of TRIM65 deletion mutants generated in pEGFP-C vectors. H Interaction of TRIM65 with BTG3 and its deletion mutants was determined by GST pull-down and western blotting analysis in HEK293T cells transfected with BTG3 and its deletion mutants. I Schematic diagram of BTG3 deletion mutants generated in pCMV-HA vectors.
