Fig. 1: STAT3β-deficient leukemia cells display increased colony formation potential and are less committed to the myeloid lineage. | Cell Death & Disease

Fig. 1: STAT3β-deficient leukemia cells display increased colony formation potential and are less committed to the myeloid lineage.

From: A novel function of STAT3β in suppressing interferon response improves outcome in acute myeloid leukemia

Fig. 1

A FLCs from WT and STAT3β–deficient (Δβ) mice were retrovirally transformed using a vector encoding MLL-AF9. B Western blot analysis of MLL-AF9 transformed FLCs with the indicated antibodies. C Growth curves of MLL-AF9 transformed FLCs (n = 6, 3 independent experiments, 2 cell lines). D Colony formation assay of WT and STAT3β-deficient leukemia cells (n = 8, 3 independent experiments with 3 serial replatings). E Representative pictures of colonies after 7 days in methylcellulose (40× magnification). F Immunophenotyping of leukemia cells after 7 days in methylcellulose via flow cytometry and representative dot plots (n = 8, 3 experiments, 3 serial replatings). Statistical analysis was performed using Student’s t test and indicated as p ≤ 0.05:*, ≤0.01:**. Error bars represent means ± SD.

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