Fig. 1: Screening of anticancer metabolism compound Library for synthetic lethality in HCT116 isogenic cells. | Cell Death & Disease

Fig. 1: Screening of anticancer metabolism compound Library for synthetic lethality in HCT116 isogenic cells.

From: Therapeutic targeting of ARID1A-deficient cancer cells with RITA (Reactivating p53 and inducing tumor apoptosis)

Fig. 1

A Loss of ARID1A expression in two HCT116 ARID1A−/− clones was verified by western blot analysis. B Schematic illustration of the synthetic lethality screening procedure. HCT116 ARID1A+/+ and ARID1A−/− #1 cell lines were screened in parallel with a 237 anticancer metabolism compound library in an 8-dose titration format. After incubation with the drug library for 72 h, cell viability was determined by Alamar Blue assay. C Scatter plot of the log2-IC50 for screening results. A log10 scale of IC50 values of the drugs against HCT116 ARID1A+/+ and ARID1A−/− cells was plotted. D A bar graph depicting potential synergistic lethal compounds selected based on the selectivity index. SI = IC50ARID1A(+/+)/ IC50ARID1A(−/−). Among all the compounds tested, the top 5 candidates are shown in the graph. E Structural formula of RITA. F Dose-response curves of HCT116 ARID1A+/+ and ARID1A−/− #1 cell lines treated with RITA.

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