Fig. 2: In vitro and in vivo synthetic lethality in HCT116 ARID1A−/− cells by RITA.
A Dose-response curves of HCT116 ARID1A+/+ and two ARID1A−/− cell lines treated with RITA. HCT116 ARID1A+/+, ARID1A−/− #1, and ARID1A−/− #2 clones were incubated with RITA for 72 h and the cell viability was determined by Alamar Blue assay. Error bars represent s.d. (n = 3) from three independent experiments. B, C Synthetic lethality in HCT116 ARID1A−/− cells by RITA. Representative images (B) and cell viability (C) are shown. HCT116 ARID1A+/+ and two ARID1A−/− clones were treated with 150 nM RITA for 48 h and photographed under an Olympus microscope. Scale bar, 200 μm. Error bars represent s.d. from three independent experiments. ANOVA **P < 0.01. D Overexpression of ARID1A in two RKO ARID1AOE clones was verified by western blot analysis. E Dose-response curves of RKO ARID1A−/− and two RKO ARID1AOE cell lines treated with RITA. RKO ARID1A−/−, ARID1AOE #1, and ARID1AOE #2 clones were incubated with RITA for 72 h and the cell viability was determined by Alamar Blue assay. Error bars represent s.d. (n = 3) from three independent experiments. F, G Synthetic lethality in RKO ARID1AOE cells by RITA. Representative images (F) and cell viability (G) are shown. RKO ARID1A−/− and two ARID1AOE clones were treated with 20 μM RITA for 48 h and photographed under Olympus microscope. Scale bar, 100 μm. Error bars represent s.d. from three independent experiments. ANOVA **P < 0.01. H Schematic illustration of mouse tumor xenograft experiments with HCT116 ARID1A−/− cell pair. I, J Tumor growth curve in nude mice bearing HCT116 ARID1A+/+ (I) or HCT116 ARID1A−/− (J) xenografts after injection of vehicle or 10 mg kg−1 (mpk) RITA. Error bars represent **P < 0.01 between vehicle and RITA treatment groups (n = 5). Student’s t-test. K, L Wet weight measurement of the tumors isolated from mice bearing HCT116 ARID1A+/+ (K) or HCT116 ARID1A−/− (L) xenografts at 21 days after injection of vehicle, 10 mpk RITA. Error bars represent s.d.
