Fig. 1: NGF is highly expressed in advanced osteosarcoma tissues and promotes cell migration. | Cell Death & Disease

Fig. 1: NGF is highly expressed in advanced osteosarcoma tissues and promotes cell migration.

From: Targeting nerve growth factor-mediated osteosarcoma metastasis: mechanistic insights and therapeutic opportunities using larotrectinib

Fig. 1

A, B NGF and TrkA family members mRNA expression in osteosarcoma tissue was analyzed in samples from the GDC data portal. C, D Normal bone and different stages of osteosarcoma specimens were stained with NGF antibody (scale bar 100 μm) for IHC, followed by quantification. E–J Osteosarcoma cells were incubated with different concentrations of NGF (30–100 ng/mL) for 18 or 24 h and cell migration and invasion were examined by the wound healing and the Transwell assay (scale bar 500 μm). All experiments were repeated 3 to 5 times. *p < 0.05 compared with the control group.

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