Fig. 3: The MEK pathway mediates NGF-induced MMP-2 expression and cell motility. | Cell Death & Disease

Fig. 3: The MEK pathway mediates NGF-induced MMP-2 expression and cell motility.

From: Targeting nerve growth factor-mediated osteosarcoma metastasis: mechanistic insights and therapeutic opportunities using larotrectinib

Fig. 3

A–F Osteosarcoma cells were incubated with MEK inhibitors (PD9059 and U0126) for 30 min or transfected with a MEK siRNA for 24 h, then stimulated with NGF, and cell migration and invasion were examined by the wound healing and the Transwell assay (scale bar 500 μm). G Cells were treated with MEK inhibitors for 30 min or transfected with a MEK siRNA for 24 h, then stimulated with NGF for 24 h. The levels of MMP-2 mRNA expression were examined by qPCR assays. H, I Cells were transfected with a MEK siRNA for 24 h, and levels of MEK protein expression were examined by Western blot. J, K Osteosarcoma cells were incubated with NGF for the indicated time intervals; MEK phosphorylation was examined by Western blot. All experiments were repeated 3 to 5 times. *p < 0.05 compared with the control group; #p < 0.05 compared with the NGF-treated group.

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