Fig. 4: The ERK pathway is involved in NGF-induced osteosarcoma MMP-2 expression and cell migration. | Cell Death & Disease

Fig. 4: The ERK pathway is involved in NGF-induced osteosarcoma MMP-2 expression and cell migration.

From: Targeting nerve growth factor-mediated osteosarcoma metastasis: mechanistic insights and therapeutic opportunities using larotrectinib

Fig. 4

A–F Osteosarcoma cells were treated with an ERK inhibitor for 30 min or transfected with an ERK siRNA for 24 h. Subsequently, the cells were stimulated with NGF, and cell migration and invasion were examined using the wound healing and the Transwell assay (scale bar 500 μm). G Cells were incubated with ERK inhibitors for 30 min or transfected with an ERK siRNA for 24 h, followed by stimulation with NGF for 24 h. The levels of MMP-2 mRNA expression were examined using qPCR assays. H, I Cells were transfected with an ERK siRNA for 24 h, and the levels of ERK protein expression were examined using Western blot. J, K Osteosarcoma cells were incubated with NGF for the indicated time, and the phosphorylation of ERK was examined using Western blot. L, M Osteosarcoma cells were pretreated with MEK inhibitors for 30 min and then stimulated with NGF for 15 min; the phosphorylation of ERK was examined using Western blot. All experiments were repeated 3 to 5 times. *p < 0.05 compared with the control group; #p < 0.05 compared with the NGF-treated group.

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