Fig. 6: Larotrectinib suppresses NGF-induced osteosarcoma cells migration in vitro. | Cell Death & Disease

Fig. 6: Larotrectinib suppresses NGF-induced osteosarcoma cells migration in vitro.

From: Targeting nerve growth factor-mediated osteosarcoma metastasis: mechanistic insights and therapeutic opportunities using larotrectinib

Fig. 6

A–C 143B cells were transfected with an empty vector or an NGF-overexpressing vector (143B/NGF). The overexpression efficiency was detected by immunoblotting. D–H The proliferation, migration, and invasion of 143B and 143B/NGF osteosarcoma cells were analyzed using colony formation, CCK-8 assay, wound healing, and the Transwell assays (scale bar 500 μm). I 143B/NGF cells were incubated with different concentrations of larotrectinib (10, 30, 100 μM) for 24 and 48 h, and cell viability was measure cell viability using MTT. J–O Osteosarcoma cells were treated with larotrectinib for 30 min. Following this, the cells were stimulated with NGF, and cell migration and invasion were assessed using the wound healing assay and the Transwell assay (scale bar 500 μm). All experiments were repeated 3 to 5 times. *p < 0.05 compared with the 143B group.

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