Fig. 6: Autophagy inhibition by 3-MA treatment inactivated the Smad2/3, PI3K/Akt, and ERK pathways in HPMCs. | Cell Death & Disease

Fig. 6: Autophagy inhibition by 3-MA treatment inactivated the Smad2/3, PI3K/Akt, and ERK pathways in HPMCs.

From: Autophagy caused by oxidative stress promotes TGF-β1-induced epithelial-to-mesenchymal transition in human peritoneal mesothelial cells

Fig. 6

3-MA treatment (2 mM) decreased the TGF-β1 (2 and 5 ng/mL)-induced phosphorylation of Smad2/3 signaling for EMT (A, B), PI3K and Akt signaling for autophagy (A, C), and the ERK signaling of the MAPK pathway (A, D). The data are presented as mean ± standard error (SE). n = 4 per group. *P < 0.05 vs. control; **P < 0.01 vs. control; ***P < 0.001 vs. control; #P < 0.05 vs. TGF-β1 2 ng/mL; ##P < 0.01 vs. TGF-β1 2 ng/mL; +P < 0.05 vs. TGF-β1 5 ng/mL; ++P < 0.01 vs. TGF-β1 5 ng/mL; and +++P < 0.001 vs. TGF-β1 5 ng/mL.

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