Fig. 2: Low expression of RRAD promotes energy status and malignant progression in OSCC cell lines. | Cell Death & Disease

Fig. 2: Low expression of RRAD promotes energy status and malignant progression in OSCC cell lines.

From: The enhanced energy metabolism in the tumor margin mediated by RRAD promotes the progression of oral squamous cell carcinoma

Fig. 2

A, B Transfection efficiency was detected using PCR and Western blot after RRAD-specific siRNA transfection for 48 h in HN4 and CAL27 cells. **P < 0.01. C, D Intracellular Ca2+ concentration, glucose uptake ability, lactate production and ADP:ATP ratio were detected after silencing RRAD in HN4 and CAL27 cell lines. *P < 0.05, **P < 0.01. E The real-time ECAR was analyzed by the Seahorse method after silencing RRAD. **P < 0.01. F A wound-healing assay was conducted to detect the migration ability of OSCC cells after silencing RRAD. Scale bar: 250 µm. G, H Migration and invasion abilities were studied using a Transwell assay after silencing RRAD in HN4 and CAL27 cells. Scale bar: 250 µm. I, J The CCK-8 and EdU assays were performed to detect proliferation ability after silencing RRAD in HN4 and CAL27 cells. Scale bar: 250 µm, *P < 0.05, **P < 0.01, ***P < 0.001.

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