Fig. 2: Pgc-1α repression leads to changes in photoreceptor and inner retinal function.
A Representative scotopic flash ERG traces at the stimulus intensity of 10 cd s/m2 between WT, Pgc-1α+/−, and Pgc-1α−/− mice (n = 5). B Line graph quantification of scotopic a-wave amplitudes between WT (n = 15), Pgc-1α+/− (n = 15), and Pgc-1α−/− (n = 10) mice at seven increasing stimulus intensities ranging from 0.001–10 cd s/m2. Black stars represent statistical significance between WT vs. Pgc-1α−/−; red represents Pgc-1α+/− vs Pgc-1α−/−, and blue represents WT vs. Pgc-1α+/−. C Quantification of a-wave amplitudes at the stimulus intensity of 10 cd s/m2. D Line graph quantification of scotopic b-wave amplitudes between WT (n = 15), Pgc-1α+/− (n = 15), and Pgc-1α−/− (n = 10). Black stars represent statistical significance between WT vs. Pgc-1α−/−, red represents Pgc-1α+/− vs. Pgc-1α−/−, and blue represents WT vs. Pgc-1α+/−. E Quantification of b-wave amplitudes at the stimulus intensity of 10 cd s/m2. F Quantification of a- and b-wave implicit times, respectively, at the stimulus intensity of 10 cd s/m2. Both male and female mice were included in the study. Two-way repeated measures ANOVA with post hoc Sidak correction for multiple comparisons was used for statistical analysis. Data are presented as mean ± SEM. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001, n.s. denotes no statistical significance.
