Fig. 7: PGC1A deficiency in ARPE19 cells downregulates cardiolipin synthesis.

A mRNA levels of cardiolipin synthesis pathway-related genes CDS1, PGS1, PTPMT1, CRLS, and TAFAZZIN (n = 3). B, C Western blot analysis of PGS1, PTPMT1, CRLS1, and TAFAZZIN protein levels (n = 3). ImageJ was used for densitometry analysis. D PGC1A inhibition in ARPE19 cells significantly decreased cardiolipin content compared to control cells (n = 3). ImageJ was used for densitometry analysis. Unpaired t-test was performed for statistical analysis. Graph represents mean ± SEM, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.