Fig. 2: Mutant Δ133p53α R273H fails to promote DNA repair but retains anti-apoptotic functions. | Cell Death & Disease

Fig. 2: Mutant Δ133p53α R273H fails to promote DNA repair but retains anti-apoptotic functions.

From: Distinct functions of wild-type and R273H mutant Δ133p53α differentially regulate glioblastoma aggressiveness and therapy-induced senescence

Fig. 2

A Fold change of RAD51 mRNA expression measured by Taqman. n = 3. B The frequency of homologous recombination was measured on cells treated with DMSO (control) or TMZ (50 μM) 24 h after treatment beginning and expressed as fold change of the control-DMSO sample for each cell line. n = 3. C γ-H2AX staining was performed on cells treated with DMSO (control) or TMZ (50 μM for 5 days). The percentage of cells with at least 10 foci was determined (corresponding images in Fig. S2B). n = 3. D Cells were grown in the presence of annexin-V dye in Incucyte® and the percentage of annexin-V staining was determined after 80 h. n = 4. E Quantification of the percentage of cells positive for Cleaved Caspases 3 by immunofluorescence (corresponding images in Fig. S2C). n = 3. F, G Bax and PUMA mRNA expression was measured by Taqman. n = 3.

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