Fig. 3: Mutant Δ133p53α R273H increases cell growth and cellular invasion. | Cell Death & Disease

Fig. 3: Mutant Δ133p53α R273H increases cell growth and cellular invasion.

From: Distinct functions of wild-type and R273H mutant Δ133p53α differentially regulate glioblastoma aggressiveness and therapy-induced senescence

Fig. 3

A Cells were imaged every 4 h in Incucyte® over 5 days and the percentage of confluence was measured. n = 4. B Cells were seeded at low density and left growing for 6 days before SRB staining was performed to determine cell growth. n = 8. C Protein expression of N-cadherin and Vimentin by Western blot. GAPDH was used as a loading control. n = 3. D Transwell assay was used to determine the percentage of invading cells 16 h after seeding. n = 5. E Cell spheroids were imaged every 4 h in Incucyte® over 6 days and the spheroid surface was measured. n = 4. F Spheroids were embedded in Matrigel and imaged every 4 h in Incucyte® over 4 days. The total spheroid and invasion area were measured. In the pictures, the yellow or blue line shows the spheroid core size, while the green line shows the limits of the spheroid total size/invasion. Invasion thus corresponds to the surface between the blue and the green lines. n = 8.

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