Fig. 2: The substantial impact of MAL2 knockdown in hindering ICC cell proliferation, invasion, and migration. | Cell Death & Disease

Fig. 2: The substantial impact of MAL2 knockdown in hindering ICC cell proliferation, invasion, and migration.

From: MAL2 reprograms lipid metabolism in intrahepatic cholangiocarcinoma via EGFR/SREBP-1 pathway based on single-cell RNA sequencing

Fig. 2

A Comparative analysis of MAL2’s mRNA expression levels in HUCCT1 and RBE cells following transfection with either sh-NC (negative control) or sh-MAL2 (sh1, sh2, and sh3). B Quantification of MAL2 protein expression in HUCCT1 and RBE cells post-transfection with sh-NC or sh-MAL2 (sh1, sh2, and sh3) using western blotting technique. C Relative mRNA and protein expression of MAL2 in HUCCT1 and RBE cells transfected with control (negative control) or MAL2 vector. D Growth kinetics of HUCCT1 and RBE cells, post-transfection with sh-NC, sh-MAL2, control or MAL2 vector, was charted based on the CCK-8 assay data at various time points – 0, 24, 48, and 72 h. E, F Cell proliferation in ICC cells post-transfection with sh-NC, sh-MAL2, control, or MAL2 vector was evaluated using an EdU assay; the scale bar represents 50 μm. G, H A wound healing assay assessed the migratory capacity of HUCCT1 and RBE cells post-transfection with sh-NC, sh-MAL2, control, or MAL2 vector; the scale bar represents 50 μm. I, J Invasion potential of HUCCT1 and RBE cells post-transfection with sh-NC, sh-MAL2, control, or MAL2 vector was investigated using a Transwell invasion assay; the scale bar stands for 200 μm. *P < 0.05; **P < 0.01; ***P < 0.001.

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