Fig. 3: APOO KO exacerbated lipid profile but had no effect on macrophage-to-feces reverse cholesterol transport (mRCT).

A–E Eight-week-old male Apoo^−/− and Apoo^+/+ controls were randomly grouped and fed an NCD (n = 7–8) or HFD (n = 8) for 12 weeks, plasma lipid profiles were evaluated. A Plasma cholesterol, B LDL-C, C HDL-C, D Triglyceride levels, E The pooled plasma from HFD-fed mice was subjected to fast protein liquid chromatography (FPLC) analysis, while cholesterol was measured in each eluted fraction. F Eight-week-old female Apoo^−/− and Apoo^+/+ controls were fed an HFD for 12 weeks, plasma cholesterol, LDL-C, HDL-C, and triglyceride levels were evaluated (n = 7–8). G Eight-week-old male Apoo^−/− and Apoo^+/+ controls were fed an HCD for 12 weeks, plasma cholesterol, LDL-C, HDL-C, and triglyceride levels were evaluated (n = 7–8). H Time course of ³H-cholesterol distribution in the plasma of 8-week-old Apoo^+/+ and Apoo^−/− mice provided with NCD. ³H-cholesterol–labeled Raw267.4 cells were injected intraperitoneally (5 × 10⁵ cpm/mice). Mice were bled at 4, 24, and 48 h after injection. Values are presented as mean ± SEM. Two-way ANOVA (A–E, H) or unpaired two-tailed Student’s t-test for other panels. *p < 0.05, **p < 0.01, ***p < 0.001. NCD normal chow diet, ANOVA, analysis of variance, SEM standard error of the mean, APOO apolipoprotein O, LDL-C lipoprotein cholesterol, HDL-C high-density lipoproteins cholesterol, HFD high-fat diet.