Fig. 5: APOO deficiency decreased hepatobiliary cholesterol and phospholipid excretion.
From: Apolipoprotein O modulates cholesterol metabolism via NRF2/CYB5R3 independent of LDL receptor
Fecal cholesterol (A) and bile acid loss (B) in 10-week-old male NCD-fed Apoo−/− mice and their control littermates (n = 6). C Schedule of [3H]-cholesterol excretion determination in vivo. Radioactivity in plasma (D), tissues (E), and feces (F) after intravenous injection of 3H-cholesterol-enriched LDL in 10-week-old male NCD-fed Apoo−/− mice and their control littermates (n = 5). G Apoo mRNA expression in the liver, heart, and BAT tissues from Apoofl/fl and AlbCreApoofl/fl mice (n = 3). H Western blot of APOO in the livers of Apoofl/fl and AlbCreApoofl/fl mice. (I) Schedule of bile sampling in vivo. Bile flow (J), hepatobiliary cholesterol (K), hepatobiliary bile acid (L), and phospholipid (M) output in male Apoofl/fl and AlbCreApoofl/fl mice fed an HFD for 12 weeks (n = 8). Values are represented as mean ± SEM. Two-tailed Student’s t-test (A, B, G) or two-way ANOVA for other panels. *p < 0.05, ***p < 0.001. ANOVA analysis of variance, SEM standard error of the mean, APOO apolipoprotein O, HFD high-fat diet, LDL low-density lipoprotein.
