Fig. 10: HOXD10 overexpression reduced ferroptosis, renal EMT and fibrosis in UUO mice.
From: HOXD10 attenuates renal fibrosis by inhibiting NOX4-induced ferroptosis
A, B The effect of AAV–HOXD10 on the mRNA expression of GPX4 and SLC7A11 in the renal cortical tissues was detected using qRT-PCR analysis. C The effect of AAV–HOXD10 on the protein expression of 4-HNE, GPX4, SLC7A11, COL I, COL IV, FN, E-cadherin and α-SMA in renal cortical tissues from various groups was detected using western blot analysis. D IHC detection of 4-HNE, GPX4, SLC7A11, COL I, COL IV, FN, E-cadherin and α-SMA in mice kidney tissues of various groups at the end of the study and average optical density of each index. (×400). Bar = 50 μm. In all panels, the data are representative of three independent experiments. Data are presented as the mean ± SD of each mouse (n = 10 mice in each group). **P < 0.01, NS not significant.
