Fig. 2: HOXD10 promoter hypermethylation leads to low HOXD10 expression in TGF-β1-induced HK-2 cells.
From: HOXD10 attenuates renal fibrosis by inhibiting NOX4-induced ferroptosis
A BSP results of the HOXD10 promoter hypermethylation in TGF-β1-induced HK-2 cells and normal control HK-2 cells. B qRT-PCR shows that the mRNAs of HOXD10 are downregulated in TGF-β1-induced HK-2 cells. C Western blot analysis shows that the proteins of HOXD10 are downregulated in TGF-β1-induced HK-2 cells. D qRT-PCR shows that the mRNAs of HOXD10 are recovered after treatment with demethylation drug 5-Aza for 48 h in TGF-β1-induced HK-2 cells. E Western blot analysis shows that the proteins of HOXD10 are recovered after treatment with demethylation drug 5-Aza for 48 h in TGF-β1-induced HK-2 cells. In all panels, the data are representative of three independent experiments. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, NS not significant.
