Fig. 3: Regulatory effect of HOXD10 on the NOX4 promoter.
From: HOXD10 attenuates renal fibrosis by inhibiting NOX4-induced ferroptosis
A HOXD10 binding site on the promoter of NOX4 was predicted by the JASPAR tool. B Forty-eight hours after treatment with HOXD10 overexpression plasmids in TGF-β1-induced HK-2 cells, the mRNA expression levels of HOXD10 were assessed using qRT-PCR. C Forty-eight hours after treatment with HOXD10 overexpression plasmids in TGF-β1-induced HK-2 cells, the mRNA expression levels of NOX4 were assessed using qRT-PCR. D Forty-eight hours after treatment with HOXD10 overexpression plasmids in TGF-β1-induced HK-2 cells, the protein expression levels of HOXD10 and NOX4 were assessed using western blot analysis. E Luciferase reporter analysis elucidated the influence of HOXD10 overexpression on the luciferase activity of the NOX4 promoter reporter in each group. F ChIP analysis of the HOXD10 binding site. The DNA fragment corresponding to the promoter of NOX4 enriched by HOXD10 binding was evaluated by agarose gel electrophoresis or real-time quantitative PCR. In all panels, the data are representative of three independent experiments. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, NS not significant.
