Fig. 5: HOXD10 overexpression inhibits ferroptosis, ECM accumulation and EMT in TGF-β1-induced HK-2 cells.
From: HOXD10 attenuates renal fibrosis by inhibiting NOX4-induced ferroptosis
A, B Forty-eight hours after treatment with HOXD10 overexpression plasmids in TGF-β1-induced HK-2 cells, the mRNA expression levels of GPX4 and SLC7A11 were assessed using qRT-PCR. C Forty-eight hours after treatment with HOXD10 overexpression plasmids in TGF-β1-induced HK-2 cells, the protein expression levels of 4-HNE, GPX4 and SLC7A11 were assessed using western blot analysis. D Forty-eight hours after treatment with HOXD10 overexpression plasmids in TGF-β1-induced HK-2 cells, the protein expression levels of COL I, COL IV, FN, E-cadherin and α-SMA were assessed using western blot analysis. E After transfection with the HOXD10 overexpression plasmids, cell proliferation was detected with a CCK-8 assay at 0, 24, 48 and 72 h. In all panels, the data are representative of three independent experiments. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, NS not significant.
