Fig. 6: HOXD10 overexpression inhibits NOX4 transcription, ferroptosis, ECM accumulation and EMT in erastin-induced HK-2 cells.
From: HOXD10 attenuates renal fibrosis by inhibiting NOX4-induced ferroptosis
A–C Forty-eight hours after treatment with HOXD10 overexpression plasmids in erastin-induced HK-2 cells, the mRNA expression levels of NOX4, GPX4 and SLC7A11 were assessed using qRT-PCR. D Forty-eight hours after treatment with HOXD10 overexpression plasmids in erastin-induced HK-2 cells, the protein expression levels of NOX4, 4-HNE, GPX4 and SLC7A11 were assessed using western blot analysis. E Forty-eight hours after treatment with HOXD10 overexpression plasmids in erastin-induced HK-2 cells, the protein expression levels of COL I, COL IV, FN, E-cadherin and α-SMA were assessed using western blot analysis. In all panels, the data are representative of three independent experiments. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, NS not significant.
