Fig. 8: Effect of HOXD10 overexpression on the expression of NOX4 in UUO mice.
From: HOXD10 attenuates renal fibrosis by inhibiting NOX4-induced ferroptosis
A, B The effect of AAV–HOXD10 on the mRNA expression of HOXD10 and NOX4 in the renal cortical tissues was detected using qRT-PCR analysis. C The effect of AAV–HOXD10 on the protein expression of HOXD10 and NOX4 in the renal cortical tissues was detected using western blot analysis. D IHC detection of HOXD10 and NOX4 in mice kidney tissues of each group and average optical density of each index (×400). Bar = 50 μm. E Dual immunofluorescent staining for HOXD10 and various segment-specific tubular markers as well as glomerular marker podocin in kidneys from sham group mice. The following segment-specific tubular markers were used: proximal tubule, lotus tetragonolobus lectin (LTL); distal tubule, peanut agglutinin (PNA); and collecting duct, dolichos biflorus agglutinin (DBA). Arrows indicate positive tubules with colocalization of HOXD10 and specific tubular markers. Bar = 50 μm. In all panels, the data are representative of three independent experiments. Data are presented as the mean ± SD of each mouse (n = 10 mice in each group). **P < 0.01, NS not significant.
