Fig. 1: FOXQ1 is upregulated in sorafenib-resistant strains.

A Images of naive SK-Hep1 and sorafenib-resistant SK-Hep1-R cells. B Evaluation of sorafenib treatment sensitivity of sorafenib-resistant cells and parental cells by clone formation assay. C The survival rate of sorafenib-resistant cells was evaluated by CCK-8. D The mRNA expression of FOXD1, FOXM1 and FOXQ1 in sorafenib-resistant cells and parental cells. E Representative IHC images of FOXQ1 expression are shown in sorafenib-sensitive and sorafenib-resistant clinical HCC samples (t-test) (n = 27), Scale bar= 20 μm. The relationship between FOXQ1 expression and prognosis in 30 sorafenib users was analyzed by the Kaplan-Meier Plotter database (http://kmplot.com/analysis/). (F: Overall survival, G: Recurrence free survival, The red line represents the survival curve of patients with high FOXQ1 expression, and the black line represents the survival curve of patients with low FOXQ1 expression. HR: Hazard ratio). **, P < 0.01; ***, P < 0.001.