Fig. 3: Phosphorylation of Serine 248 affects the regulation of ferroptosis by FOXQ1. | Cell Death & Disease

Fig. 3: Phosphorylation of Serine 248 affects the regulation of ferroptosis by FOXQ1.

From: Phosphorylated FOXQ1, a novel substrate of JNK1, inhibits sorafenib-induced ferroptosis by activating ETHE1 in hepatocellular carcinoma

Fig. 3

A Effect of sorafenib (10 μM) on phosphorylation of FOXQ1 at serine, tyrosine and threonine sites. The numbers between the strips are grayscale values calculated by imageJ. B Effect of sorafenib (10 μM) treatment on the phosphorylation of Flag-FOXQ1 serine sites (Flag-FOXQ1 was transfected into cells instantaneously). C Effect of sorafenib (10 μM) on phosphorylation of FOXQ1 at serine 248 (S248). D The effect of the mutation at Serine 248 on the phosphorylation of FOXQ1. SK-Hep1 and PLC/PRF/5 cells were transiently transfected with Vector, FOXQ1 and FOXQ1S248A, then the iron content (E, F) and lipid peroxidation (G, H) were assessed by flow cytometry after DMSO and sorafenib (10 μM) treatment. P values were determined by Student’s t-test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

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