Fig. 6: FOXQ1 activates the transcription of ETHE1 in HCC cells.

A FOXQ1 ChIP-Seq was performed on PLC/PRF/5 cells treated with DMSO or sorafenib (10 μM) for 24 h, and the Venn diagram showed the ChIP-seq results of FOXQ1 in each group. B PLC/PRF/5 cells were transiently transfected with Vector and FOXQ1. Real-time quantitative polymerase chain reaction(qRT-PCR) to verify the effect of FOXQ1 expression on the expression of GLS2, NOX4 and ETHE1 after treatment with DMSO and sorafenib (10 μM). C–E SK-Hep1 and PLC/PRF/5 cells were transiently transfected with Vector, FOXQ1 and FOXQ1^S248A, then the cells were treated with DMSO or sorafenib (10 μM) for 24 h, the effects of FOXQ1^WT and FOXQ1^S248A on the expression of ETHE1 were detected by real-time quantitative polymerase chain reaction (qRT-PCR) and western blotting. F ChIP-Seq results were validated by qChIP analysis of the indicated genes. After DMSO or sorafenib (10 μM) treatment for 24 h, PLC/PRF/5 cells were collected, and qChIP experiments were performed with the indicated antibodies. G PLC/PRF/5 cells were co-transiently transfected with FOXQ1^WT or FOXQ1^S248A and the luciferase reporter gene driven by the ETHE1 promoter (ETHE1-Luc), the luciferase activity was detected after treatment with DMSO or sorafenib (10 μM) for 24 h. Relative luciferase activity was calculated by dividing firefly luciferase activity by renilla luciferase activity and is shown relative to controls. *, P < 0.05; **, P < 0.01; ***, P < 0.001.