Fig. 5: M1 macrophage of Trim26−/− mice facilitates liver regeneration by secreting more Wnts. | Cell Death & Disease

Fig. 5: M1 macrophage of Trim26−/− mice facilitates liver regeneration by secreting more Wnts.

From: TRIM26 deficiency enhancing liver regeneration through macrophage polarization and β-catenin pathway activation

Fig. 5

A Bone marrow-derived macrophages or THP-1 were induced M0, M1, and M2 polarization, respectively. Conditioned media were collected and applied to primary hepatocytes or L02 cells. B, C Primary hepatocytes were treated with different CMs, and cell cycle assay were measured by flow cryometry. D The relative expression of Wnts was detected in M0, M1, and M2 macrophages derived from the bone marrow of Trim26−/− and WT mice. E Wnts mRNA expression was detected in M0, M1, and M2 macrophages from control and Flag-Trim26 THP-1 cells. F Primary hepatocytes were treated with different CMs from BMDM, respectively. And the protein expression of β-catenin signaling proteins were measured. G, H L02 treated with CMs from THP-1, respectively, and the protein expression of β-catenin signaling proteins were measured. Representative data and average of triplicate repeats are shown. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

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