Fig. 6: Bone marrow transplantation mitigated the promoting effect of Trim26 deficiency on liver regeneration. | Cell Death & Disease

Fig. 6: Bone marrow transplantation mitigated the promoting effect of Trim26 deficiency on liver regeneration.

From: TRIM26 deficiency enhancing liver regeneration through macrophage polarization and β-catenin pathway activation

Fig. 6

A WT and Trim26−/− mice were exposed to 8 Gy of γ radiation, and the bone marrow was injected into the mice through the tail vein. After 8 weeks, mice were treated with CCl4 for two days. B, C Representative HE staining and Ki67 staining and the statistical quantification. (n = 5, scale bar, 50 µm). D, E Serum ALT and AST levels. F, G Representative FACS plots and the statistical quantification of hepatic macrophage (F4/80+CD11b+) profiles of CD45+ liver monocytes from Trim26−/− and WT mice on second day after CCl4 treatment. n = 4–6/group. H Representative graphs of flow cytometry analysis of primary hepatocytes cell cycle, and the statistical analysis of percentages of cells at different cell cycle stages (G0/G1, S and G2/M). n = 7–10/group. I, J mRNA levels and protein expression of cell cycle proteins and β-catenin signaling were examined after bone marrow transplantation. n = 7–10/group. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

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